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Features​

  • Flow rates from 1 to 150 mL/min allows analytical scouting and Prep methods on one system
  • Operating pressure up to 6,000 psi
  • Choice of UV or UV-Vis plus ELSD and MS options
  • One touch Focus Gradient Generator​ minimizes purification time while maximizing sample recovery
  • Complete control of the system on one screen
  • Lifetime of free software upgrades​​​​​
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Improved Resolution is One Touch Away!

Save time and solvent while increasing column load​​ing.

Designed to allow quick method optimizatio​​n to get to your purified product by every chemist. Run a single scouting run, then use our exclusive Focus Gradient feature, where you are a touch of the peak away from an optimized gradient that maximizes efficiency and resolution around your target compound. This method is automatically scaled up to the column size (of matching media) of choice. Seamless integration with our ​PurIon MS confirms you have selected the right peak to optimize.​​​​.​

Scouting Run vs Focused Run
Scouting Run (left): Run a scouting run on the ACCQPrep HP150 or from a calibrated analytical system and simply touch the peak of interest or enter its retention time for an optimized method for scale up. Focused Run (right): Observe the improved resolution allowing increased sample loading and increasing throughput with minimal method development time.

Focus Gradient Generator paired with the PurIon mass spectrometer

Easy to Use Meth​​od Optimization Right the First Time!

No more guessing which peak is your compound! Coupling a scouting run with MS data means you can now have confidence that the separation is optimized for your target compound. Getting things done right the first time, saves money, solvent and most importantly time.


Learn More about PurIon​​​

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Use of Focused Gradient Generator and PurIon MS to scale up purification of peptide from 4.6 x 150 mm column to 20 x 150 mm column​.

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No Chromophore? No Problem with our ELSD and MS modules!

Integrated ​ELSD

Collect weak UV absorbing comp​​ounds with certainty.
  • Fully integrated into the ACCQPrep HP150 with no additional bench space needed.
  • Offers scalability and sensitivity for a wide range of sample loading with easy-tochange method parameters.
  • Peak alignment automatically adjusted for varying solvent mixturesand different flow rates.
  • Alignment from 2 to 150 mL/min with no user-hardware changes.
ELSD graphs
top to bottom: 4.6 x150 @ 2 mL/min | 20 x150 @ 20 mL/min | 30 x250 @ 42.5 ​mL/min

​Instruction Sheets

​Preparative High ​​Pressure Liquid Chromatography System

Evolving to become more recently known as high performance liquid chromatography, HPLC systems work faster and at higher pressures than some other types of liquid chromatography systems, in part because the mobile phase can be introduced under greater pressure than with other techniques. This makes HPLC systems an excellent choice for qualitative and quantitative analysis. HPLC can also be used for the preparative purification of compounds, resulting in preparative high performance liquid chromatography systems like the ACCQPrep HP150.

Frequently A​​sked Questions 

What is liquid chromato​graphy?

​​In liquid chromatography, a solvent known as the mobile phase is used to transport a mixture of compounds through a column containing a stationary phase, most generally consisting of silica gel (leading to the term “gel permeation chromatography”). Liquid chromatography is a technique that can be used for quantitative analysis and for preparative purifications as well.  

What is the diffe​rence between high performance liquid chromatography and flash chromatography?​​​​

In high performance liquid chromatography, the mobile phase is pushed through the stationary phase under higher pressure than that used for flash chromatography. The higher pressure is a result of using a stationary phase with smaller particle size than that used for flash chromatography resulting in increased resolution.  HPLC systems therefore yield faster results and HPLC separation to a superior ability to distinguish between compounds and elute purer results. 

What does high pressu​re liquid chromatography do?

Chromatography is essential to research, development, purification, analysis, and manufacturing across many industries: pharmaceutical analysis; the analysis of bodily fluids in drug tests; separating fats, oils, sugars, vitamins, and natural coloring agents in foods; cosmetics and personal care products; preservatives of all kinds; and to develop and purify color compounds, including paints and dyes. 

The separation of the compounds in the mixture is based on the affinity of each component’s molecules for the mobile phase and the stationary phase. The varied mass of the molecules and their varying propensity to adsorb, or adhere to, the stationary phase causes the compounds to migrate at different speeds. The results can then be separated out into their individual components using a fraction collector.